Tehran University of Medical Sciences Immunology and Genetics Journal 2645-4831 4 3 2021 09 22 155 160 Sara Hanaei Department of Neurosurgery, Imam Khomeini Hospital Complex (IKHC), Tehran University of Medical Sciences (TUMS), Tehran, Iran Golshid Sanati Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal Scientific Education and Research Network (USERN), Tehran, Iran Maryam Sadr Molecular Immunology Research Center, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Safoora Gharibzadeh Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran Roham Salmanroghani School of Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran Hassan Salmanroghani Department of Gastrointestinal Diseases, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran Nima Rezaei Tehran University of Medical Sciences 2022 12 29 2023 02 27 Background and Aims: Comprised of two main subtypes (Ulcerative Colitis (UC) and Crohn), inflammatory bowel disease is caused by an interaction between genetic and environmental factors. As of the important role of innate immunity and JAK/STAT signaling pathway, the current study was designed to investigate the methylation status JAK2 gene in blood and tissue samples of patients with UC. Methods: Genomic DNA was extracted from blood and intestinal biopsy samples of 28 UC patients and 28 controls. After bisulfite DNA conversion, real-time quantitative multiplex methylation specific PCR (QM-MSP) method was applied in order to assess JAK2 promotor methylation status. Results: The JAK2 promotor in the intestinal biopsy samples was significantly hypermethylated in UC as the mean of unmethylated DNA was 1.255±1.865 in the patients group, while it was 1.292±4.726 in control group (P=0.002). Conclusions: Hypermathylation of JAK2 gene may play a part in pathophysiology of UC which could result in gene silencing. https://igj.tums.ac.ir/index.php/igj/article/view/112 https://igj.tums.ac.ir/index.php/igj/article/download/112/79